Perio-Analyse ®

The colonization of the mouth by periodontal bacteria can take place at an early age of the patient but can also take place after dental implant placement (peri-implantitis) if the patient was not being properly treated against pathogenic bacteria or having poor oral hygiene.


Biological analysis accessible to practitioners
Perio-Analyse – identification and quantification of periodontal-pathogenic species using real-time PCR and Cell culture.

Diagnosis and Prevention
Periodontal disease – diagnosis, choice of treatment and maintenance.
Peri-implantitis – risk prevention, analytical support for treatment, forensic control and assistance.

Real Time PCR
Microbial DNA extraction from paper points. DNA amplification with DNA probes and primers.
Multiparametric quantification of DNA bacterial specific sequences from gingival crevicular fluid or peri-implant sulcular fluid

Microflora to be tested – Quantification of the following organisms

Aggregatibacter actinomycetemcomitans
A.a. is sensitive to New Quinolone and Tetracycline, but is practically insensitive to Metronidazol. There is a strong relation with the youthful periodontitis (very high).

Bone lost could be more than 3 mm in 2 months with only 105 bacteria into the pocket (Haffajee 1994).

Antibiotics are always necessary and surgical plasty could be recommended in some cases to eliminate soft tissue. A.a. is transmissible in particular between parents and children.

Porphyromonas gingivalis (if present at a certain quantity)
Porphyromonas is sensitive to Tetracycline and Penicillin.

Bone lost could be more than 2mm in 2 months with only 105 bacteria into the pocket (Haffajee 1994).

P.g. is transmissible between parents and children and between partners.

Prevalence of Peri-implantitis
28-56% of patients have peri-implantitis at one or more implants
(Zitzmann & Berglunch, 2008)

Prevalence of bone loss
16% of patients / 6.6% of the implants have ≥1.8mm bone loss after 1 year of implantation. (Roos-Jansaker et al., 2006a)
28% of patients have progressive bone loss over 5 years of post-implantation.
(Fransson et al., 2005)

Bone Loss
Dependent on type of bacteria and quantity in CFU (colony forming unit)

Benefits for the practitioner:

  • Diagnostic aid
  • Choice of treatment and antibiotic therapy
  • Increase patient’s motivation
  • Risk mitigation peri-implantitis
  • Biological support of dental service